CHARACTERIZATION OF A POLYCLONAL ANTIPEPTIDE ANTIBODY TO THE ANGIOTENSIN-II TYPE-1 (AT1) RECEPTOR

被引：42

作者：

ZELEZNA, B

RICHARDS, EM

TANG, W

LU, D

SUMNERS, C

RAIZADA, MK

机构：

[1] Department of Physiology, College of Medicine University of Florida, Gainesville

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90551-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polyclonal antibody has been prepared against a synthetic peptide corresponding to amino acids 14-23 of the angiotensin II type-1 (AT1) receptor. The antibody is of high titer and mono-specific. Western blot analysis of membranes from rat liver, kidney, and adrenal gland showed that the antibody specifically recognizes a protein band of MW 70,000 whose amounts are highest in the liver, followed by kidney and adrenals. In addition, a relatively less prominent band of MW 95,000 was also detected. The relative distribution of this protein correlates well with the values obtained for [3H]-DuP753 binding and AT1 receptor mRNA. © 1992.

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页码：781 / 788

页数：8

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