INTERLEUKIN-10 IN HUMAN-MILK

被引：141

作者：

GAROFALO, R ^{[1
]}

CHHEDA, S ^{[1
]}

MEI, F ^{[1
]}

PALKOWETZ, KH ^{[1
]}

RUDLOFF, HE ^{[1
]}

SCHMALSTIEG, FC ^{[1
]}

RASSIN, DK ^{[1
]}

GOLDMAN, AS ^{[1
]}

机构：

[1] UNIV TEXAS, MED BRANCH, DEPT PEDIAT, GALVESTON, TX 77555 USA

来源：

PEDIATRIC RESEARCH | 1995年 / 37卷 / 04期

关键词：

D O I：

10.1203/00006450-199504000-00010

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

The concentrations of immunoreactive IL-10 in the aqueous fraction of 20 specimens of human milk obtained during the first 80 h of lactation and stored at -60 degrees C ranged from 66 to 9301 pg/mL (mean +/- SD, 3304 +/- 3127 pg/mL). IL-10 was present also in the lipid layer of milk. Gel filtration revealed that IL-10 was located in a high molecular weight fraction, where certain other cytokines in human milk have been found. In addition, immunoreactive IL-10 in milk increased after treatment with sodium taurocholate, Bioactive IL-10 was demonstrated by the finding that human milk inhibited [H-3]thymidine uptake by human blood lymphocytes and that inhibition was partly overcome by concomitant incubation with antibodies to human IL-10. IL-10 mRNA but no protein product was found in cultured human mammary epithelial cells. Some IL-10 was associated with preparations of human milk leukocytes, but the data did not suggest that the cells were producing the cytokine. Bioactive IL-10 in a possible protected compartment suggests that IL-10 in human milk may have immunomodulating, antiinflammatory effects on the alimentary tract of the recipient infant.

引用

页码：444 / 449

页数：6

共 44 条

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