AN ANALYTICAL MODEL OF IONIC MOVEMENTS IN AIRWAY EPITHELIAL-CELLS

A new mathematical model of ion movements in airway epithelia is presented, which allows predictions of ion fluxes, membrane potentials and ion concentrations. The model includes sodium and chloride channels in the apical membrane, a Na/K pump and a cotransport system for Cl-with stoichiometry Na+: K+: 2Cl-in the basolateral membrane. Potassium channels in the basolateral membrane are used to regulate cell volume. Membrane potentials, ion fluxes and intracellular ion concentration are calculated as functions of apical ion permeabilities, the maximum pump current and the cotransport parameters. The major predictions of the model are: (1) Cl-concentration in the cell is determined entirely by the intracellular concentration of negatively charged impermeable ions and the osmotic conditions; (2) changes in intracellular Na+and K+concentrations are inversely related; (3) cotransport provides the major driving force for Cl-flux, increases intracellular Na+concentration, decreases intracellular K+concentration and hyperpolarizes the cell interior; (4) the maximum rate of the Na/K pump, by contrast, has little effect on Na+or Cl-transepithelial fluxes and a much less pronounced effect on cell membrane polarization; (5) an increase in apical Na+permeability causes an increase in intracellular Na+concentration and a significant increase in Na+flux; (6) an increase in apical Cl-permeability decreases intracellular Na+concentration and Na+flux; (7) assuming Na+and Cl-permeabilities equal to those measured in human nasal epithelia, the model predicts that under short circuit conditions, Na+absorption is much higher than Cl-secretion, in agreement with experimental measurements. © 1991 Academic Press Limited.