ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING THE ANTIVIRAL PROTEIN FROM PHYTOLACCA-AMERICANA

被引:83
作者
LIN, Q
CHEN, ZC
ANTONIW, JF
WHITE, RF
机构
[1] Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, AL5 2JQ, Herts
关键词
CDNA; PHYTOLACCA ANTIVIRAL PROTEIN; RIBOSOME-INACTIVATING PROTEINS; SEQUENCING;
D O I
10.1007/BF00037047
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.
引用
收藏
页码:609 / 614
页数:6
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