CONSIDERATIONS IN THE DEVELOPMENT OF A SENSITIVE HPLC ASSAY FOR HUMAN EPIDERMAL GROWTH-FACTORS IN HUMAN PLASMA

被引：5

作者：

KAGEL, JR

ROSSI, DT

NORDBLOM, GD

DUDECK, RC

BARKSDALE, CM

KUO, BS

WRIGHT, DS

机构：

[1] Department of Pharmacokinetics and Drug Metabolism, Parke-Davis Pharmaceutical Research, Divison of Warner-Lambert Co., Ann Arbor

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1995年 / 13卷 / 10期

关键词：

TANDEM IMMUNOAFFINITY AND SCX HPLC; TRACE QUANTITATION; DIRECT PLASMA INJECTION; EPIDERMAL GROWTH FACTOR;

D O I：

10.1016/0731-7085(95)01561-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive assay was developed for human epidermal growth factors (hEGF) 1-48 (dosed), hEGF 1-53 (endogenous), without interference from potential metabolites hEGFs 1-47 or 1-46. Spiked human plasma samples were injected directly, utilizing on-line immunoaffinity HPLC (anti-hEGF) clean-up. No change in capacity was noted after 81 cycles. After release from the immunoaffinity column, the fragments were further resolved by strong cation-exchange (SCX) via a column switching valve. Method development also required interfacing immunoaffinity, ion-exchange, and detection components. Immunoassays on collected fractions yielded a detection limit of 1 mu g ml(-1), although a detection limit of 75 pg ml(-1) appears feasible.

引用

页码：1205 / 1213

页数：9

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