NA+-SUGAR COTRANSPORT SYSTEM AS A POLARIZATION MARKER DURING ORGANIZATION OF EPITHELIAL MEMBRANE

被引:20
作者
LASHERAS, C
SCOTT, JA
RABITO, CA
机构
[1] MASSACHUSETTS GEN HOSP, DEPT RADIOL, DIV NUCL MED, BOSTON, MA 02114 USA
[2] HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1988年 / 255卷 / 06期
关键词
D O I
10.1152/ajpcell.1988.255.6.C745
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The present study analyzed the changes in Na+-dependent sugar transport and transepithelial electrical resistance as LLC-PK1 cells reorganize into epithelial membranes. Sugar influx increased to reach a maximum 9 h after plating. The increase in the transepithelial electrical resistance, however, showed a significant delay, reaching steady state 15 h after plating. No changes in the electrochemical Na+ gradient were observed during the reorganization of the epithelial membranes. Kinetic analysis and pH phlorizin-binding studies showed that the increase in sugar influx resulted from an increase in the number of carriers. Unidirectional sugar influx measurements indicated that the sugar transporters were primarily located at the apical surface of the epithelial cells. These observations are consistent with the hypothesis that the sorting of native proteins occurs intracellularly before their insertion in the apical membrane, or as an alternative that they are randomly inserted, but then immediately sorted such as any carrier could be detected in the basolateral side during the reorganization process. In addition, the results suggest that the functional developement of the apical membrane may occur before the complete sealing of the intercellular space during the development of the sugar transport system and occluding junctions was inhibited by cycloheximide and nitomycin but not by actinomycin D, suggesting that the expression of epithelial cell polarizaiton is probably a posttranslational event in the protein synthesis.
引用
收藏
页码:C745 / C753
页数:9
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