RESTRICTION ENDONUCLEASE ANALYSIS OF CHROMOSOMAL DNA FROM LISTERIA-MONOCYTOGENES STRAINS

被引：11

作者：

CASOLARI, C

FACINELLI, B

FABIO, U

ROCOURT, J

VARALDO, PE

机构：

[1] Cattedra di Microbiologia, Universita di Modena, Modena, 41100, Via Campi

[2] Istituto di Microbiologia, Universitá di Ancona, Ancona

[3] Unité d'Ecologie Bactérienne, Institut Pasteur, Paris Cedex 15

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1990年 / 6卷 / 03期

关键词：

DNA fingerprinting; L; monocytogenes; Restriction enzymes;

D O I：

10.1007/BF00150440

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Restriction endonuclease analysis of chromosomal DNA was applied to thirteen Listeria monocytogenes strains alongside the more conventional typing methods of serotyping and phage typing. The organisms were isolated from cases of sporadic listeriosis (nine strains); from an occasional nosocomial cluster (two strains); and from food samples (two strains). Purified DNAs were digested with EcoRI restriction endonuclease, and restriction fragments separated by electrophoresis. Restriction patterns correlated well with phage patterns, but also allowed typing of the phage-untypable strains. DNA fingerprinting appears to be a potentially helpful tool for epidemiological investigations of listeric infections, particularly when phage typing fails to determine the identity or diversity of the isolates. © 1990 Kluwer Academic Publishers.

引用

页码：319 / 322

页数：4

共 15 条

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