THERMOSTABILITY AND ELECTRON-TRANSFER ACTIVITY OF THE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII

被引：5

作者：

AONO, S ^{[1
]}

FUKUDA, N ^{[1
]}

OKURA, I ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT BIOENGN,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL | 1995年 / 95卷 / 02期

关键词：

FERREDOXIN; IRON-SULFUR CLUSTER; BACILLUS SCHLEGELII;

D O I：

10.1016/1381-1169(94)00009-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The Azotobacter-type 7Fe ferredoxin from Bacillus schlegelii was active as an electron carrier in the reduction system of cyt. c consisting of NADPH, FNR and cytochrome c (cyt, c). The only [3Fe-4S] cluster in the Fd molecule acts as the active site for the reduction of cyt, c. The activity of the ferredoxin once increased by 10, 20 and 20% by heat treatment at 60, 80 and 90 degrees C, respectively. The increasing of the activity is caused by the interconversion of the [4Fe-4S] cluster to the [3Fe-4S] cluster with increasing of the [3Fe-4S] cluster in the concentration.

引用

页码：173 / 178

页数：6

共 19 条

[1] CLONING AND EXPRESSION OF THE GENE ENCODING THE 7FE TYPE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII
AONO, S
NAKAMURA, S
AONO, R
OKURA, I
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 201 (02) : 938 - 942
[2] PURIFICATION AND CHARACTERIZATION OF A 7FE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII
AONO, S
KURITA, H
UNO, S
OKURA, I
[J]. JOURNAL OF BIOCHEMISTRY, 1992, 112 (06) : 792 - 795
[3] ARMSTRONG FA, 1988, FEBS LETT, V107, P107
[4] STRUCTURE, FUNCTION AND EVOLUTION OF BACTERIAL FERREDOXINS
BRUSCHI, M
GUERLESQUIN, F
[J]. FEMS MICROBIOLOGY LETTERS, 1988, 54 (02) : 155 - 175
[5] PURIFICATION, CHARACTERIZATION AND BIOLOGICAL-ACTIVITY OF 3 FORMS OF FERREDOXIN FROM SULFATE-REDUCING BACTERIUM DESULFOVIBRIO-GIGAS
BRUSCHI, M
HATCHIKIAN, EC
LEGALL, J
MOURA, JJG
XAVIER, AV
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 449 (02) : 275 - 284
[6] INTERCONVERSIONS BETWEEN THE 3FE AND 4FE FORMS OF THE IRON-SULFUR CLUSTERS IN THE FERREDOXIN FROM THERMODESULFOBACTERIUM-COMMUNE - ELECTRON-PARAMAGNETIC-RES CHARACTERIZATION AND POTENTIOMETRIC TITRATION
GUIGLIARELLI, B
BERTRAND, P
MORE, C
PAPAVASSILIOU, P
HATCHIKIAN, EC
GAYDA, JP
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 810 (03) : 319 - 324
[7] MYCOBACTERIUM-SMEGMATIS FERREDOXIN - UNIQUE DISTRIBUTION OF CYSTEINE RESIDUES CONSTRUCTING IRON-SULFUR CLUSTERS
HASE, T
WAKABAYASHI, S
MATSUBARA, H
IMAI, T
MATSUMOTO, T
TOBARI, J
[J]. FEBS LETTERS, 1979, 103 (02) : 224 - 228
[8] PSEUDOMONAS-OVALIS FERREDOXIN - SIMILARITY TO AZOTOBACTER AND CHROMATIUM FERREDOXINS
HASE, T
WAKABAYASHI, S
MATSUBARA, H
OHMORI, D
SUZUKI, K
[J]. FEBS LETTERS, 1978, 91 (02) : 315 - 319
[9] IRON-SULFUR CLUSTERS AND CYSTEINE DISTRIBUTION IN A FERREDOXIN FROM AZOTOBACTER VINELANDII
HOWARD, JB
LORSBACH, T
QUE, L
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 70 (02) : 582 - 588
[10] JOHNSON MK, 1984, J BIOL CHEM, V259, P2274

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