MEASUREMENTS OF FISH GILL ATPASES USING MICROPLATES

被引：16

作者：

MAYERGOSTAN, N ^{[1
]}

LEMAIRE, S ^{[1
]}

机构：

[1] UNIV PERPIGNAN,BIOL MARINE LAB,F-66025 PERPIGNAN,FRANCE

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 98卷 / 2-3期

关键词：

D O I：

10.1016/0305-0491(91)90185-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. The activities of enzymes involved in ion transport, namely Na+K+-ATPase and high affinity Ca2+-ATPase, were determined in membranes isolated from fish gills by measuring the inorganic phosphate produced by hydrolysis of ATP. 2. The incubation and colour reaction obtained in the presence of malachite green reagent were developed in 96-well microplates. 3. The novel microassay procedure is sensitive and reliable and allows for more detailed analysis of enzyme properties to be conducted, particularly when the amount of biological material is a limiting factor.

引用

页码：323 / 326

页数：4

共 11 条

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