UV DAMAGE AND REPAIR IN THE DOMAIN OF THE HUMAN C-MYC ONCOGENE

被引：10

作者：

BIANCHI, NO

BIANCHI, MS

ALITALO, K

DELACHAPELLE, A

机构：

[1] UNIV HELSINKI,DEPT VIROL,SF-00100 HELSINKI 10,FINLAND

[2] UNIV HELSINKI,DEPT MED GENET,SF-00100 HELSINKI 10,FINLAND

来源：

DNA AND CELL BIOLOGY | 1991年 / 10卷 / 02期

关键词：

D O I：

10.1089/dna.1991.10.125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a modification of the Southern hybridization method to analyze the removal of the UV-induced pyrimidine cyclobutane dimers from the domain of the c-myc oncogene. The study was performed in human COLO320HSR cells, which exhibit a 30- to 40-fold amplification of c-myc that is maintained in a marker chromosome as a homogeneously staining region. Intron 2 and the region upstream from the gene showed better dimer removal than intron 1 or the region downstream from the c-myc gene. Regions showing less repair coincide with regions that are hotspots for mutations and chromosome translocations. Therefore, it is proposed that the inefficiency of DNA repair may play an important role in the origin of c-myc rearrangements.

引用

页码：125 / 132

页数：8

共 22 条

[1] HOMOGENEOUSLY STAINING CHROMOSOMAL REGIONS CONTAIN AMPLIFIED COPIES OF AN ABUNDANTLY EXPRESSED CELLULAR ONCOGENE (C-MYC) IN MALIGNANT NEUROENDOCRINE CELLS FROM A HUMAN-COLON CARCINOMA [J].