CLONING AND CHARACTERIZATION OF AN ALPHA-AMYLASE GENE FROM STREPTOMYCES-LIVIDANS

被引：13

作者：

TSAO, LS ^{[1
]}

LIN, LL ^{[1
]}

CHEN, JC ^{[1
]}

CHEN, JH ^{[1
]}

HSU, WH ^{[1
]}

机构：

[1] FOOD IND RES & DEV INST,CULTURE COLLECT & RES CTR,HSINCHU,TAIWAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1171卷 / 03期

关键词：

ALPHA-AMYLASE; AMY GENE; (STREPTOMYCES-LIVIDANS);

D O I：

10.1016/0167-4781(93)90063-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The alpha-amylase gene (amy) of Streptomyces lividans TK24 was cloned in an amylase deficient mutant strain S. lividans M2. The cloned gene contained an open reading frame (ORF) of 2757 nucleotides (919 amino acids) coding for a protein of 100 kDa. Sequencing of the amino terminus of the extracellular alpha-amylase protein revealed the presence of a signal peptide of 33 amino acid residues. The transcriptional initiation site was mapped by the primer extension method with T4 DNA polymerase and was found to be transcribed from an unique promoter. The alpha-amylase protein produced by S. lividans was larger than those derived from other origins. It also contained the four common conserved regions characteristic of other alpha-amylase proteins.

引用

页码：255 / 262

页数：8

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