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GLUCOSE-OXIDASE AS AN ANALYTICAL REAGENT

被引：169
作者：
RABA, J
MOTTOLA, HA
机构：
[1] OKLAHOMA STATE UNIV,DEPT CHEM,STILLWATER,OK 74078
[2] UNIV NACL SAN LUIS,FAC QUIM BIOQUIM & FARM,DEPT QUIM ANALIT,RA-5700 SAN LUIS,ARGENTINA
来源：
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY | 1995年 / 25卷 / 01期
关键词：
ENZYMES AS ANALYTICAL REAGENTS; GLUCOSE OXIDASE;
D O I：
10.1080/10408349508050556
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Glucose oxidase (EC 1.1.3.4) is the most widely employed enzyme as analytical reagent. This is the result of (1) its utility in the determination of glucose, an analyte of wide analytical interest, and (2) its relatively low cost and good stability that make the glucose/glucose oxidase system a very convenient model for method development (particularly in the area of biosensors). This review discusses in detail enzyme structure, biocatalysis, enzymes as analytical reagents, properties of glucose oxidase (including a historical account), and the use of glucose oxidase as an analytical reagent in homogeneous systems as well as an immobilized reagent.
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页码：1 / 42
页数：42
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