SOLUBLE MULTIMERIC ALZHEIMER BETA(1-40) PRE-AMYLOID COMPLEXES IN DILUTE-SOLUTION

被引：87

作者：

LEVINE, H

机构：

[1] Department of Neurodegenerative Diseases, Parke-Davis Pharmaceutical Research Division, the Warner-Lambert Company, Ann Arbor, MI 48106-1047

来源：

NEUROBIOLOGY OF AGING | 1995年 / 16卷 / 05期

关键词：

GLUTARALDEHYDE; SYNTHETIC PEPTIDE; FIBRIL FORMATION MODEL; CROSS-LINKING; BOROHYDRIDE REDUCTION; SDS-PAGE; MULTIMERS; PH DEPENDENCE;

D O I：

10.1016/0197-4580(95)00052-G

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Aqueous solutions of beta(1-40) peptide spontaneously associate to form pentameric/hexameric complexes that can be demonstrated by SDS-PAGE following treatment with glutaraldehyde and borohydride reduction. Under amyloidogenic conditions of pH and high peptide concentration these aggregates can further associate to form sedimentable and filterable structures with beta-sheet amyloid characteristics of Thioflavine T fluorescence. The presence of such preamyloid structures at low peptide concentration suggests a mechanism by which amyloid plaques can accrete additional material by a cooperative rather than monomeric growth. The existence of a monomer <-> multimer equilibrium may partly explain the divergence of biological consequences with respect to neurotoxicity.

引用

页码：755 / 764

页数：10

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