A NOVEL THERMOTOLERANT METHYLOTROPHIC BACILLUS SP AND ITS POTENTIAL FOR USE IN SINGLE-CELL PROTEIN-PRODUCTION

被引：6

作者：

BANAT, IM

MURAD, M

HAMDAN, IY

机构：

[1] Department of Biology, University of Al-Ain, Al-Ain

[2] Kuwait Institute for Scientific Research, Safat, 13109

[3] Agriculture Research Station, Jordan University of Science and Technology, Irbid

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 03期

关键词：

BACILLUS; METHYLOTROPHIC BACTERIUM; METHYLOTROPHS; SINGLE-CELL PROTEIN; THERMOTOLERANT;

D O I：

10.1007/BF01201882

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A thermotolerant methylotrophic Bacillus sp. (KISRI TM1A, NCIMB 40040), isolated from the Kuwaiti environment and belonging to the group II spore-forming bacilli, could not be correlated with any known Bacillus sp. It may, therefore, be a new species. It grew at temperatures from 37-degrees to 58-degrees-C, from pH 6.5 to 9.0 and on methanol up to 40 g l-1. It grew well in a chemostat. Its biomass yield coefficient was improved by about 30% by optimization of medium and growth conditions, reaching a maximum of 0.44 g g-1 at 45-degrees-C, pH 6.8 to 7.0, dilution rate 0.25 h-1 with methanol at 10 g l-1. Average crude protein and amino acid content were 84% and 60%, respectively, and maximum productivity attained under laboratory conditions was 5.06 g l-1h-1. It was concluded that this strain has good potential for use in single-cell protein production.

引用

页码：290 / 295

页数：6

共 22 条

[1]

Abu-Ruwaida A.S., Banat I.M., Hamdan I.Y., Chemostat optimization of biomass production of a mixed bacterial culture utilizing methanol, Applied Microbiology and Biotechnology, 32, pp. 550-555, (1990)

[2]

Al-Awadhi N., Asthana H.N., Hamdan I.Y., Research and development activities for SCP production by thermophilic methanol utilizing cultures in Kuwait, Proceedings of the International Symposium on SCP from Hydrocarbons, pp. 221-226, (1983)

[3]

Al-Awadhi N., Egli T., Hamer G., Growth charcteristics of a thermotolerant methylotrophic Bacillus sp. (NCIB 12522) in batch culture, Applied Microbiology and Biotechnology, 29, pp. 485-493, (1988)

[4]

Al-Awadhi N., Egli T., Hamer G., Thermotolerant and thermophilic solvent-utilizing methylotrophic aerobic bacteria, Systematic and Applied Microbiology, 11, pp. 207-216, (1989)

[5]

Al-Awadhi N., Hamer G., Egli T., The bio-oxidation of methanol, ethanol and isopropanol by a defined co-culture at elevated temperatures, Bioprocess Engineering, 5, pp. 39-45, (1990)

[6]

Arfman N., Dijkhuizen L., Methanol dehydrogenase from the thermotolerant methylotroph, Bacillus sp. C1, Methods in Enzymology, 188, pp. 223-226, (1990)

[7]

Arfman N., Watling E.M., Clement W., Van Oosterwijk R.J., De Vries G.E., Harder W., Attwood M.M., Dijkhuizen L., Methanol metabolism in thermotolerant methylotrophic Bacillus strains involving a novel catabolic NAD-dependent methanol dehydrogenase as a key enzyme, Archives of Microbiology, 152, pp. 280-288, (1989)

[8]

Banat I.M., Al-Awadhi N., Hamdan I.Y., Physiological characteristics of four methylotrophic bacteria and their potential use in single cell protein production, MIRCEN Journal of Applied Microbiology and Biotechnology, 5, pp. 207-216, (1989)

[9]

Bull A.T., Brown C.M., Continuous culture application to microbial biochemistry, International Review of Biochemistry and Microbiology, pp. 177-226, (1979)

[10]

Dijkhuizen L., Arfman N., Attwood M.M., Brooke A.G., Harder W., Watling E.M., Isolation and initial characterization of thermotolerant methylotrophic Bacillus strains, FEMS Microbiology Letters, 52, pp. 209-214, (1988)

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