HUMAN ENDOTHELIN ET(A) RECEPTOR ANTISENSE OLIGODEOXYNUCLEOTIDES INHIBIT ENDOTHELIN-1 EVOKED VASOCONSTRICTION

被引：14

作者：

ADNER, M

ERLINGE, D

SALFORD, LG

YEE, F

WAHLESTEDT, C

EDVINSSON, L

机构：

[1] UNIV LUND HOSP,DEPT INTERNAL MED,S-22185 LUND,SWEDEN

[2] UNIV LUND HOSP,DEPT NEUROSURG,S-22185 LUND,SWEDEN

[3] CORNELL UNIV,COLL MED,DEPT NEUROL & NEUROSCI,DIV NEUROBIOL,NEW YORK,NY

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1994年 / 261卷 / 03期

关键词：

ANTISENSE OLIGODEOXYNUCLEOTIDE; ENDOTHELIN ET(A) RECEPTOR; TEMPORAL ARTERY; HUMAN;

D O I：

10.1016/0014-2999(94)90118-X

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Antisense oligodeoxynucleotides to endothelin ET(A) receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ET(B) receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ET(A) receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA(2) = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ET(A) receptors mediating contraction in the human superficial temporal artery.

引用

页码：281 / 284

页数：4

共 14 条

[1] CHARACTERIZATION OF ENDOTHELIN-A RECEPTORS IN THE CEREBRAL-CIRCULATION [J].