METHOD FOR 3D VOLUMETRIC-ANALYSIS OF INTRANUCLEAR FLUORESCENCE DISTRIBUTION IN CONFOCAL MICROSCOPY

被引：24

作者：

PARAZZA, F

HUMBERT, C

USSON, Y

机构：

[1] Equipe de Reconnaissance des Formes et Microscopie Quantitative, Laboratoire TIM3-IMAG, USR CNRS 69013, CERMO, Université Joseph Fourier

来源：

COMPUTERIZED MEDICAL IMAGING AND GRAPHICS | 1993年 / 17卷 / 03期

关键词：

3D ANALYSIS; IMAGE PROCESSING; CONFOCAL MICROSCOPY; DNA REPLICATION;

D O I：

10.1016/0895-6111(93)90043-M

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The introduction of the confocal laser scanning microscope makes it possible to acquire fluorescent specimens in 3D. We present basic image processing tools to enhance the data and to quantitate the morphology and topography of the intravolume elements. The tools were applied to the description of the spatial distribution of DNA replication sites in mammalian cell nuclei as an example.

引用

页码：189 / 200

页数：12

共 21 条

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