PURIFICATION AND CHARACTERIZATION OF PECTINESTERASE PRODUCED BY A STRAIN OF ASPERGILLUS-NIGER

被引：31

作者：

MALDONADO, MC

DESAAD, AMS

CALLIERI, D

机构：

[1] Department of Industrial Microbiology, Faculty of Biochemistry and Pharmacy, National University of Tucumán, Tucumán

来源：

CURRENT MICROBIOLOGY | 1994年 / 28卷 / 04期

关键词：

D O I：

10.1007/BF01575960

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

After an 88-fold purification, pectinesterase produced by a strain of Aspergillus niger, isolated from rotten lemons, showed the following main characteristics: maximum activity at 45 degrees C, pH 5; Km, with pectin as substrate, 1.01 mg/L; Delta G(*), 4750 Cal/mol. Polygalacturonic acid and methanol acted as competitive and non-competitive inhibitors, respectively. The activity of the enzyme was impaired by MgCl2 and stimulated by NaCl.

引用

页码：193 / 196

页数：4

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