TRANSCRIPTION AND REGULATION OF THE CPDB GENE IN ESCHERICHIA-COLI K12 AND SALMONELLA-TYPHIMURIUM LT2 - EVIDENCE FOR MODULATION OF CONSTITUTIVE PROMOTERS BY CYCLIC AMP-CRP COMPLEX

Measurements of cyclic phosphodiesterase, or of β-galactosidase in the case of cpdB′-′ZacZ fusions, indicate that cpdB expression in both Escherichia coli and Salmonella typhimurium is modulated by carbon source availability, consistent with previous observations in Salmonella. Nucleotide sequence analysis and transcription mapping of both cpdB genes have revealed, in their 5′ flanking regions, sequences with good similarity to consensus -10 and -35 regions and cyclic AMP-cyclic AMP receptor protein (cAMP-CRP) binding sites. Furthermore, they are strongly conserved in both organisms. Deletion analysis of an E. coli cpdB′-′ lacZ fusion supports the identification of these elements, and a role for the cAMP-CRP binding site in modulating constitutive cyclic phosphodiesterase expression. © 1990 Springer-Verlag.