AN UPSTREAM PROMOTER ELEMENT OF THE ACANTHAMOEBA-CASTELLANII TBP GENE BINDS A DNA-SEQUENCE SPECIFIC TRANSCRIPTION ACTIVATING PROTEIN, TPBF

被引：17

作者：

LIU, F ^{[1
]}

BATEMAN, E ^{[1
]}

机构：

[1] UNIV VERMONT,DEPT MICROBIOL & MOLEC GENET,BURLINGTON,VT 05405

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 18期

关键词：

D O I：

10.1093/nar/21.18.4321

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized a positive-acting element in the upstream portion of the Acanthamoeba TBP gene promoter. The 27 bp element (TPE), located within the promoter between -97 and -70, stimulates transcription in an orientation independent fashion and tolerates modest changes i n its distance from the TATA box. The TPE does not, however, function synergistically nor when positioned 3000 bp 5' or 260 base pairs 3' of the transcription start site. The TPE binds a DNA sequence-dependent factor, TPBF, which we have partly purified. TPBF was characterized using in vitro transcription, DNase I footprinting, methylation interference and electrophoretic mobility shift assays. TPBF does not have a counterpart in HeLa cells, but nonetheless strongly stimulates transcription of the Acanthamoeba TBP gene in mammalian extracts. Our results also suggest that there are additional positively and negatively acting elements within the TBP gene promoter, for which a model is presented.

引用

页码：4321 / 4329

页数：9

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