CHARACTERIZATION OF 5-HT3 RECEPTORS IN INTACT N1E-115 NEUROBLASTOMA-CELLS

被引：47

作者：

LUMMIS, SCR

KILPATRICK, GJ

MARTIN, IL

机构：

[1] MRC,MOLEC NEUROBIOL UNIT,CAMBRIDGE CB2 2QH,ENGLAND

[2] GLAXO GRP RES LTD,DEPT NEUROPHARMACOL,WARE SG12 0DP,HERTS,ENGLAND

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1990年 / 189卷 / 2-3期

关键词：

5-HT3; RECEPTOR; N1E-115 NEUROBLASTOMA CELLS; GR65630;

D O I：

10.1016/0922-4106(90)90026-T

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The highly selective 5-HT3 receptor antagonist [H-3]GR65630 has been used to characterize 5-HT3 receptors in intact N1E-115 neuroblastoma cells. Equilibrium binding analysis demonstrated high-affinity binding to a single class of receptors with a K(d) of 0.69 (+/- 0.12) nM and B(max) of 31.4 (+/- 11.4) fmol/10(5) cells, equivalent to approximately 200 000 sites per cell. Specific binding was displaced by low concentrations of 5-HT3-selective ligands, and by the nicotinic antagonist d-tubocurarine.

引用

页码：223 / 227

页数：5

共 10 条

[1] 5-HT3 RECEPTORS ARE MEMBRANE ION CHANNELS [J].