EXOGENOUS TRANSFORMING GROWTH-FACTOR-BETA AMPLIFIES ITS OWN EXPRESSION AND INDUCES SCAR FORMATION IN A MODEL OF HUMAN FETAL SKIN REPAIR

被引：142

作者：

LIN, RY ^{[1
]}

SULLIVAN, KM ^{[1
]}

ARGENTA, PA ^{[1
]}

MEULI, M ^{[1
]}

LORENZ, HP ^{[1
]}

ADZICK, NS ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, DEPT SURG, SAN FRANCISCO, CA 94143 USA

来源：

ANNALS OF SURGERY | 1995年 / 222卷 / 02期

关键词：

D O I：

10.1097/00000658-199508000-00006

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Objective Fetal skin wounds heal without scarring. To determine the role of IGF-beta(1) in fetal wound healing, mRNA expression of TGF-beta(1) was analyzed in human fetal and adult skin wounds. Methods Human fetal skin transplanted to a subcutaneous location on an adult athymic mouse that was subsequently wounded heals without scar, whereas human adult skin heals with scar formation in that location. In situ hybridization for TGF-beta(1) mRNA expression and species-specific immunohistochemistry for fibroblasts, macrophages, and neutrophils were performed in human adult wounds, fetal wounds, and fetal wounds treated with a TGF-beta(1) slow release disk. Results Transforming growth factor-beta(1) mRNA expression was induced by wounding adult skin. No TGF-beta(1) mRNA upregulation was detected in human fetal skin after wounding. However, when exogenous TGF-beta(1) was added to human fetal skin, induction of TGF-beta(1) mRNA expression in human fetal fibroblasts occurred, an adult-like inflammatory response was detected, and the skin healed with scar formation. Conclusions Transforming growth factor-beta(1) is an important modulator in scar formation. Anti-TGF-beta(1) strategies may promote scarless healing in adult wounds.

引用

页码：146 / 154

页数：9

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