STRUCTURE IDENTIFICATION OF FREE-RADICALS BY ESR AND GC MS OF PBN SPIN ADDUCTS FROM THE INVITRO AND INVIVO RAT-LIVER METABOLISM OF HALOTHANE

Free radicals were detected from the in vim metabolism of halothane (rat liver microsomes) by the PBN spin trapping method. The detected radical species include the I-chloro-2.2.2-trifluoro-I-ethyl radical (I). as determined by mass spectral analysis, and lipid-type radicals assigned by high resolution ESR spectro-scopy with the use of d, deuterated PBN. The lipid-derived radicals are a carbon-centred radical with the partially assigned structure 'CH, R and an oxygen-centred radical of the OR type. From the mass spectral analysis of the spin adduct mixture there is also evidence for a halocarbon double adduct of PBN of the type I-PBN-I. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.