DEVELOPMENTAL EXPRESSION OF CHOLINE-ACETYLTRANSFERASE MESSENGER-RNA IN DROSOPHILA

被引：14

作者：

CARBINI, LA ^{[1
]}

MAINES, VJM ^{[1
]}

SALVATERRA, PM ^{[1
]}

机构：

[1] CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV NEUROSCI,DUARTE,CA 91010

来源：

NEUROCHEMICAL RESEARCH | 1990年 / 15卷 / 11期

关键词：

CHOLINE ACETYLTRANSFERASE; DEVELOPMENT; MESSENGER RNA; DROSOPHILA;

D O I：

10.1007/BF01101709

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have measured the steady state levels of choline acetyltransferase (ChAT, EC 2.3.1.6) mRNA during different developmental stages of Drosophila melanogaster using a ChAT specific cRNA probe. ChAT mRNA was first detected approximately 6-7 h after oviposition, increased until the 1st-2nd larval instar, decreased into early pupal stage and increased again during late pupation, reaching a maximum in adults. Northern analysis showed a major RNA band with a Mr of 4.7 kilobases and Western analysis also showed a single major 75 kD protein band at all developmental stages. Our results support the hypothesis that a major point of regulation of ChAT expression may be at the transcriptional level.

引用

页码：1089 / 1096

页数：8

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