LIGHT AND ELECTRON-MICROSCOPE IMMUNOCYTOCHEMICAL ANALYSES OF HISTONE H1(0) DISTRIBUTION IN THE NUCLEUS OF FRIEND-ERYTHROLEUKEMIA CELLS

被引：19

作者：

GORKA, C ^{[1
]}

FAKAN, S ^{[1
]}

LAWRENCE, JJ ^{[1
]}

机构：

[1] UNIV LAUSANNE, CTR MICROSCOPIE ELECTR, CH-1005 LAUSANNE, SWITZERLAND

来源：

EXPERIMENTAL CELL RESEARCH | 1993年 / 205卷 / 01期

关键词：

D O I：

10.1006/excr.1993.1069

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The localization of histone H10 in murine erythroleukemia cells which were induced to resume a differentiation program was studied in cells which have recovered their proliferative capacity after transient blockage in the G1 phase of the cell cycle. Previous studies have shown that histone H10 accumulation occurs at early times of induction and is probably related to the commitment itself. The distribution of the protein was determined by immunomicroscopy with monoclonal antibodies specific for histone H10. Our observations showed that the histone accumulates in nuclei. Immunoeclectron microscopy further demonstrated the presence of histone H10 in condensed chromatin areas, including perinucleolar chromatin. Moreover, histone H10 also occurred in the perichromatin regions, previously described as preferential sites of pre-mRNA synthesis, suggesting that histone H10 is not fully excluded from active chromatin. © 1993 Academic Press, Inc.

引用

页码：152 / 158

页数：7

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