MICROBIAL DEHALOGENATION OF TRICHLOROACETIC-ACID

被引：30

作者：

WEIGHTMAN, AL ^{[1
]}

WEIGHTMAN, AJ ^{[1
]}

SLATER, JH ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF,SCH PURE & APPL BIOL,POB 915,CARDIFF CF1 3TL,WALES

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 05期

关键词：

DEHALOGENASES; DEHALOGENATION; METHYLOTROPHS; PSEUDOMONAS; TRICHLOROACETIC ACID;

D O I：

10.1007/BF01201951

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A pure bacterial culture and a two-membered mixed culture were isolated that degraded trichloroacetic acid if a second, readily metabolizable substrate was present in the growth medium. Previous doubts over the microbial dehalogenation of trichloroacetic acid (TCA) may be due to its inability to act as a sole carbon and energy source. TCA dehalogenation was associated with conventional 2-haloalkanoic acid dehalogenases but oxalate, the putative dehalogenase product, was not detected. CO2 was produced rapidly and concomitantly with Cl- ion release during dehalogenation of TCA. An alternative mechanism is suggested for TCA dehalogenation via an initial decarboxylation reaction. This mechanism predicts that carbon monoxide is a product of TCA decarboxylation and it was significant that one of the organisms isolated, Pseudomonas carboxydohydrogens, was a carboxytroph and a second was an unidentified facultative methylotroph.

引用

页码：512 / 518

页数：7

共 26 条

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