BORDETELLA-PERTUSSIS LIPOPOLYSACCHARIDE AS ANTIGEN IN ELISA FOR SEROLOGICAL DIAGNOSIS OF WHOOPING-COUGH

被引：5

作者：

GRANSTROM, G

GRANSTROM, M

ANDERSON, M

LINDQUIST, U

LEONTEIN, K

SVENSON, SB

WRETLIND, B

机构：

[1] Department of Infectious Diseases, Danderyd Hospital

[2] Department of Vaccine Production and Development, National Bacteriological Laboratory

[3] Department of Clinical Microbiology, Karolinska Hospital, S-104 01 Stockholm

[4] Pharmacia Biosensor AB

[5] Nobel Koncernservice AB, S-100 61 Stockholm

[6] Department of Bacteriology, Danderyd Hospital

来源：

SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE | 1990年 / 4卷 / 02期

关键词：

Bordetella pertussis; diagnosis; ELISA; lipopolysaccharide; serology; whooping cough;

D O I：

10.1016/0888-0786(90)90040-U

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Bordetella pertussis lipopolysaccharide (LPS) was investigated as antigen for serological diagnosis of whooping cough by ELISA in paired samples from 90 patients with culture-confirmed pertussis. Significant IgG titre rises were seen in 70 (78%) of patients, IgM titre rises in 49 (54%) and IgA in 56 (62%). A rise in either IgG or IgM was noted in 74 (82%). In unimmunised children, IgG titre rises were seen in 63 74 (85%), while the corresponding response was noted in only 7 16 (44%) of the previously DTP-immunised children and adults. Inclusion of IgG responses to filamentous haemagglutinin (FHA) increased the diagnostic sensitivity to 96% ( 86 90 positives by titre rises). In the unimmunised group, 73 74 (99%) showed IgG titre rises to either of the antigens. Titre rises to high levels of IgM to the LPS antigen were significantly more common than to FHA or pertussis toxin (PT). In conclusion, the study showed that B. pertussis LPS can be useful as antigen in the serological diagnosis of whooping cough, especially in non-DTP immunised populations. © 1990.

引用

页码：151 / 158

页数：8

共 27 条

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