ELECTRON-PROBE MICROANALYSIS OF CALCIUM RELEASE AND MAGNESIUM UPTAKE BY ENDOPLASMIC-RETICULUM IN BEE PHOTORECEPTORS

Honey bee photoreceptors contain large sacs of endoplasmic reticulum (ER) that can be located unequivocally in freeze-dried cryosections. The elemental composition of the ER was determined by electron probe x-ray microanalysis and was visualized in high-resolution x-ray maps. In the ER of dark-adapted photoreceptors, the Ca concentration was 47.5 +/- 1.1 mmol/kg (dry weight) (mean +/- SEM). During a 3-sec nonsaturating light stimulus, almost-equal-to 50% of the Ca content was released from the ER. Light stimulation also caused a highly significant increase in the Mg content of the ER; the ratio of Mg uptake to Ca released was almost-equal-to 0.7. Our results show unambiguously that the ER is the source of Ca2+ release during cell stimulation and suggest that Mg2+ can nearly balance the charge movement of Ca2+.