EXPRESSION OF A CLOSTRIDIUM-THERMOCELLUM XYLANASE GENE IN BACILLUS-SUBTILIS

被引：15

作者：

JUNG, KH ^{[1
]}

PACK, MY ^{[1
]}

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT BIOTECHNOL,373-1 KUSONG DONG,YUSUNG GU,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1007/BF00133009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The native promoter of a xylanase gene isolated from Clostridium thermocellum was replaced with a strong promoter screened from Bacillus subtilis chromosomes. A part of the C-terminal region of the gene which is not related to the xylanase activity was removed. With the modified xylanase gene, B. subtilis was transformed and grown in LB medium. The xylanase gene was expressed well in B. subtilis and extracellular xylanase was produced up to 30 units per ml when the growth reached OD600 of 4.8.

引用

页码：115 / 120

页数：6

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