AN ONLINE SEMIAUTOMATED SOLID-PHASE EXTRACTION PROCEDURE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LONIDAMINE IN SERUM

被引：5

作者：

BOTTALICO, C

MICELLI, G

GUERRIERI, A

PALMISANO, F

LORUSSO, V

DELENA, M

机构：

[1] UNIV BASILICATA,DIPARTIMENTO CHIM,I-85100 POTENZA,ITALY

[2] UNIV BARI,DIPARTMENTO CHIM,I-70126 BARI,ITALY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1995年 / 13卷 / 11期

关键词：

SOLID-PHASE EXTRACTION; REVERSED-PHASE LIQUID CHROMATOGRAPHY; UV DETECTION; LONIDAMINE;

D O I：

10.1016/0731-7085(95)01542-S

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A semi-automated solid-phase extraction procedure on-line with gradient elution reversed-phase chromatography permits the determination of lonidamine and its metabolite in human serum. The average recovery from serum at the 2.5 mu g ml(-1) level was (92.8 +/- 3.4)%. The limit of quantitation for a 100 mu l sample size was 50 ng ml(-1) The within-day (n=5) and between-day (n=5) relative standard deviations for lonidamine determination in serum samples spiked at the 2.5 mu g ml(-1) level were 2.7% and 4.5%, respectively.

引用

页码：1349 / 1353

页数：5

共 20 条

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