CYTOTOXICITY IN-VITRO OF ORGANOTIN COMPOUNDS TO FISH HEPATOMA-CELLS PLHC-1 (POECILIOPSIS-LUCIDA)

被引：64

作者：

BRUSCHWEILER, BJ

WURGLER, FE

FENT, K

机构：

[1] SWISS FED INST ENVIRONM SCI & TECHNOL, CH-8600 DUBENDORF, SWITZERLAND

[2] ETH ZURICH, INST TOXICOL, CH-8603 SCHWERZENBACH, SWITZERLAND

[3] UNIV ZURICH, CH-8603 SCHWERZENBACH, SWITZERLAND

来源：

AQUATIC TOXICOLOGY | 1995年 / 32卷 / 2-3期

关键词：

POECILIOPSIS LUCIDA; CYTOTOXICITY; FISH HEPATOMA CELLS; ORGANOTIN COMPOUNDS; NEUTRAL RED ASSAY; MTT ASSAY; BROMODEOXYURIDINE; CRYSTAL VIOLET; IN VITRO IN VIVO COMPARISON;

D O I：

10.1016/0166-445X(94)00087-7

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Cytotoxicity in vitro to fish hepatoma cells PLHC-1 has been analyzed for a series of 21 organotin compounds consisting of all degrees of alkylation and arylation. The sensitivity of the neutral red (NR) assay and the tetrazolium salt reduction (MTT) assay was similar for most of the organotin compounds. Cytotoxic effects were found at concentrations between 10(-8) M and 10(-2) M. For various trisubstituted organotin compounds, including tributyltin and triphenyltin, which are used in antifouling paints and as pesticides, respectively, cytotoxic concentrations in the range of 10(-8) M to 10(-6) M were observed. Based on the concentration reducing NR uptake by 50% (NR(50)), the sequence of cytotoxicity amongst butyltins was tributyltin > bis(tributyl)tin > dibutyltin > tetrabutyltin > butyltin > tin(IV). Tributyltin induced effects on cell functions quickly, as a reduction in NR uptake by 30% was recorded after 30 min exposure to 4 . 10(-7) M. The ranking order in cytotoxicity in the MTT assay of phenylated organotins was triphenyltin > diphenyltin > phenyltin > tin(TV). Cytotoxic concentrations of tributyltin and triphenyltin measured in the bromodeoxyuridine (BrdU) assay and with the crystal violet (CV) staining method do not differ significantly from those determined in the NR and MTT assay. Tri-and disubstituted organotin compounds exhibit a significant correlation between the n-octanol/water partition coefficient (logK(ow)) and the NR(50) (n = 10, r = 0.86, P = 0.001) and the MTT(50) values (n = 11, r = 0.85, P = 0.001), respectively. In addition, good qualitative correlations between in vitro cytotoxicity data and in vivo fish toxicity data were found (for NR assay n = 8, r = 0.86, P = 0.001; for MTT assay n = 9, r = 0.80, P = 0.001). The results indicate that the in vitro cytotoxicity assays using PLHC-1 cells are useful tools for the estimation of the acute toxicity to fish of organotins and possibly other compounds.

引用

页码：143 / 160

页数：18

共 48 条

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