CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF HUMAN TISSUE FACTOR EXTRACELLULAR DOMAIN

被引：9

作者：

BOYS, CWG

MILLER, A

HARLOS, K

MARTIN, DMA

TUDDENHAM, EGD

OBRIEN, DP

机构：

[1] OXFORD CTR MOLEC SCI,OXFORD OX1 3QU,ENGLAND

[2] LAB MOLEC BIOPHYS,OXFORD OX1 3QU,ENGLAND

[3] CLIN RES CTR,HAEMOSTASIS RES GRP,HARROW HA1 3UJ,MIDDX,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 234卷 / 04期

基金：

英国惠康基金;

关键词：

BLOOD COAGULATION; TISSUE FACTOR; CRYSTALLOGRAPHY;

D O I：

10.1006/jmbi.1993.1678

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extracellular domain (residues 1 to 220) of human tissue factor has been cloned and expressed in Escherichia coli and purified to isoelectric homogeneity. Single crystals suitable for X-ray analysis have been obtained by vapour diffusion. They belong to the tetragonal space group P41212 or P43212 with a = b = 45.2 Å, c = 231.5 Å, contain one molecule per asymmetric unit and diffract to 2.6 Å resolution. Native and derivative data sets have been collected to 3.6 and 3.9 Å, respectively. © 1993 Academic Press Limited.

引用

页码：1263 / 1265

页数：3

共 18 条

[1] HUMAN-TISSUE FACTOR CONTAINS THIOESTER-LINKED PALMITATE AND STEARATE ON THE CYTOPLASMICHALF-CYSTINE [J].