Optimization of DNA amplification fingerprinting techniques to study genetic relationships of white lupin germplasm

被引：11

作者：

Qiu, J

VanSanten, E

Tuzun, S

机构：

[1] AUBURN UNIV, DEPT AGRON & SOILS, AUBURN, AL 36849 USA

[2] ALABAMA AGR EXPT STN, AUBURN, AL 36849 USA

来源：

PLANT BREEDING | 1995年 / 114卷 / 06期

关键词：

Lupinus albus; genetic markers; polymerase chain reaction;

D O I：

10.1111/j.1439-0523.1995.tb00849.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

DNA amplification fingerprinting (DAF) techniques were used to evaluate genetic relationships among 24 randomly selected white lupin (Lupinus albus L.) accessions originating from four endemic regions. Parameters affecting DAF were optimized to obtain high resolution and reproducible banding patterns. DNA amplification was tolerant to a wide range of both template and primer concentrations but sensitive to Mg2+ concentration, and annealing temperature and time. Pure template DNA was found to be essential for obtaining reproducible banding patterns. Primer annealing occurred at multiple sites, permitting detection of polymorphism using only a single primer. Of 56 octamer primers tested, 22 yielded highly polymorphic DAF bands that could be used to distinguish closely related accessions. Genetic similarity coefficients were calculated and a dendrogram, using the unweighted pair group method with arithmetic average (UPGMA), was constructed. A high level of genome diversity was found among the accessions, but several from the Middle East and West Africa tended to cluster together. The results support the future use of DAF markers for the characterization and identification of white lupin germplasm.

引用

页码：525 / 529

页数：5

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