ENZYME-CATALYZED ORGANIC-SYNTHESIS OF SUCROSE AND TREHALOSE WITH INSITU REGENERATION OF UDP GLUCOSE

This paper describes cell-free enzymatic syntheses of sucrose and trehalose using partially-purified preparations of sucrose and trehalose synthetase. The coupling of the regeneration of uridine-5′-diphosphoglucose (UDP-Glc) with synthesis of the disaccharide offers a practical route to millimol quantities of these carbohydrates. The syntheses used pyruvate kinase, UDP-Glc pyrophosphorylase, and inorganic pyrophosphatase, and the regenerated UDP-Glc was cycled approximately 10 times. © 1990 Humana Press Inc.