ENZYME-CATALYZED ORGANIC-SYNTHESIS OF SUCROSE AND TREHALOSE WITH INSITU REGENERATION OF UDP GLUCOSE

被引:23
作者
HAYNIE, SL
WHITESIDES, GM
机构
[1] HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138
[2] MIT,DEPT CHEM,CAMBRIDGE,MA 02139
关键词
sucrose; trehalose; UDP-Glc; regeneration of;
D O I
10.1007/BF02798384
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes cell-free enzymatic syntheses of sucrose and trehalose using partially-purified preparations of sucrose and trehalose synthetase. The coupling of the regeneration of uridine-5′-diphosphoglucose (UDP-Glc) with synthesis of the disaccharide offers a practical route to millimol quantities of these carbohydrates. The syntheses used pyruvate kinase, UDP-Glc pyrophosphorylase, and inorganic pyrophosphatase, and the regenerated UDP-Glc was cycled approximately 10 times. © 1990 Humana Press Inc.
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页码:155 / 170
页数:16
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