THICKNESS MEASUREMENTS ON LIVING CELL MONOLAYERS BY NUCLEAR METHODS

被引:14
作者
DURANTE, M
GIALANELLA, G
GROSSI, GF
PUGLIESE, M
机构
[1] UNIV NAPOLI FEDERICO 2,DIPARTIMENTO SCI FIS,MOSTRA OLTREMARE PADIGL 20,I-80125 NAPLES,ITALY
[2] IST NAZL FIS NUCL,SEZ NAPOLI,I-80125 NAPLES,ITALY
关键词
D O I
10.1016/0168-583X(93)95838-V
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Determination of the absorbed dose is essential to study the biological effects of ionizing radiation. Dosimetry requires the knowledge of cell thickness in the same conditions as used in the designed experiments. This can be obtained by measuring charged beam energy loss in the sample. We used an accelerated alpha-particle beam and CR-39 track-etch detectors or silicon surface barrier detectors to measure the energy. In the first case, cells were grown on CR-39 plastics, in the latter cells were grown on a thin mylar foil placed in front of the silicon detector. Cell thickness was obtained as the difference of ranges R(E) - R(E'), where E' and E are the measured energies with and without the cell monolayer in front of the detector, respectively. The measured thickness of C3H 10T1/2 murine fibroblasts growing as a monolayer on CR-39 plastics was 3.3 +/- 2.0 mum, and 3.6 +/- 1.9 mum when grown on mylar films. These results are compared with those obtained in the same cell line by microscopy in other laboratories. Nuclear methods provide reasonable estimates of the cell thickness on living cells, in the same conditions adopted for radiobiological experiments, and provide information on the fluctuations in the monolayer thickness. Further applications of these methods in radiation biology are mentioned.
引用
收藏
页码:543 / 549
页数:7
相关论文
共 18 条
[1]  
BIERSACK JP, 1991, TRIM91 TRANSPORT ION
[2]   RADON - CURRENT CHALLENGES IN CELLULAR RADIOBIOLOGY [J].
BRENNER, DJ .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1992, 61 (01) :3-13
[3]   NUCLEAR-TRACK-RECORDING POLYMER OF UNIQUE SENSITIVITY AND RESOLUTION [J].
CARTWRIGHT, BG ;
SHIRK, EK ;
PRICE, PB .
NUCLEAR INSTRUMENTS & METHODS, 1978, 153 (2-3) :457-460
[4]  
FLEISCHER RL, 1975, NUCLEAR TRACKS SOLID
[5]   EFFECTIVENESS OF 0.3 KEV CARBON ULTRASOFT X-RAYS FOR THE INACTIVATION AND MUTATION OF CULTURED MAMMALIAN-CELLS [J].
GOODHEAD, DT ;
THACKER, J ;
COX, R .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1979, 36 (02) :101-115
[6]  
JAMES F, 1983, CERN D506 COMP CTR P
[7]   THE PREPARATION OF BIOLOGICAL TARGETS FOR HEAVY-ION EXPERIMENTS UP TO 20 MEV/U [J].
KRAFTWEYRATHER, W ;
KRAFT, G ;
RITTER, S ;
SCHOLZ, M ;
STANTON, JA .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 1989, 282 (01) :22-27
[8]   CELL SURVIVAL FOLLOWING MULTIPLE-TRACK ALPHA-PARTICLE IRRADIATION [J].
LLOYD, EL ;
GEMMELL, MA ;
HENNING, CB ;
GEMMELL, DS ;
ZABRANSKY, BJ .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1979, 35 (01) :23-31
[9]   INACTIVATION OF C3H 10T1/2 CELLS BY MONOENERGETIC HIGH LET ALPHA-PARTICLES [J].
NAPOLITANO, M ;
DURANTE, M ;
GROSSI, GF ;
PUGLIESE, M ;
GIALANELLA, G .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1992, 61 (06) :813-820
[10]  
REZNIKOFF CA, 1973, CANCER RES, V33, P3231