GENE ACTIVITY AND CLEAVAGE ARREST IN HUMAN PREEMBRYOS

被引：72

作者：

ARTLEY, JK

BRAUDE, PR

JOHNSON, MH

机构：

[1] Assisted Conception Research Unit, Department of Obstetrics Gynaecology, United Medical and Dental School of Guy's and St Thomas' Hospitals, St. Thomas' Campus, London SE1 7EH, 6th Floor, North Wing

[2] Embryo and Gamete Research Group, University of Cambridge, Department of Anatomy, Cambridge

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 07期

基金：

英国医学研究理事会;

关键词：

CLEAVAGE ARREST; GENE EXPRESSION; HUMAN; PREEMBRYO;

D O I：

10.1093/oxfordjournals.humrep.a137761

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

There is a high rate of spontaneous cleavage arrest around the four- to eight-cell stage of human development in vitro. Since this coincides with the time of activation of the embryonic genome it has been suggested that cleavage arrest may occur as a consequence of failure of gene activation. Gene expression in human pre-embryos is associated with an alpha-amanitin sensitive, qualitative change in protein synthesis. In order to ascertain the role of gene expression in cleavage arrest, we have examined the protein synthetic patterns of human pre-embryos which have undergone spontaneous cleavage arrest in vitro. Of 54 cleavage-arrested embryos, 27 demonstrated evidence of synthesis of proteins sensitive to alpha-amanitin, suggesting that cleavage arrest is not always accompanied by failure of activation of the genome. Our results would also suggest that activation of gene expression is simply related to neither cell number nor time spent in culture since fertilization, but may be related to continuing karyokinesis.

引用

页码：1014 / 1021

页数：8

共 33 条

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