DETECTION OF LEISHMANIA RNA VIRUS-1 PROTEINS

被引：29

作者：

CADD, TL

KEENAN, MC

PATTERSON, JL

机构：

[1] CHILDRENS HOSP MED CTR,DIV INFECT DIS,300 LONGWOOD AVE,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 09期

关键词：

D O I：

10.1128/JVI.67.9.5647-5650.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

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页码：5647 / 5650

页数：4

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