HYBRID CELL EXTINCTION AND REEXPRESSION OF OCT-3 FUNCTION CORRELATES WITH DIFFERENTIATION POTENTIAL

被引:70
作者
SHIMAZAKI, T
OKAZAWA, H
FUJII, H
IKEDA, M
TAMAI, K
MCKAY, RDG
MURAMATSU, M
HAMADA, H
机构
[1] UNIV TOKYO, FAC MED, DEPT BIOCHEM, 7-3-1 HONGO, BUNKYO KU, TOKYO 113, JAPAN
[2] MBL CO LTD, INA LAB, INA, NAGANO 396, JAPAN
[3] MIT, DEPT BRAIN & COGNIT SCI, CAMBRIDGE, MA 02139 USA
关键词
EMBRYOGENESIS; HYBRID CELL EXTINCTION; OCT-3; POU;
D O I
10.1002/j.1460-2075.1993.tb06138.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Oct-3 gene is expressed in highly undifferentiated cells and is implicated in mammalian early embryogenesis. We have generated a series of hybrid cells between pluripotent embryonal carcinoma cells (Oct-3+) and fibroblasts (Oct-3-), and have studied the regulation and function of Oct-3. Upon fusion, the hybrid cells differentiated to nestin+/Brn-2+ cells resembling neuroepithelial stem cells. Expression of Oct-3 was extinguished at the transcriptional level in all the hybrid cells examined. The Oct-3 modulating activity required for the Oct-3-mediated enhancer activation was also extinguished. When the Oct-3 transactivating function was introduced into the hybrid cells, they transformed into morphologically distinct nestin-/Brn-2- cells ('revertants'). When the 'revertant' cells subsequently lost Oct-3 expression, they differentiated back to nestin+/Brn-2+ cells. The close correlation between the phenotypic changes and the gain/loss of Oct-3 function indicates that Oct-3 can induce dedifferentiation of the neural cells.
引用
收藏
页码:4489 / 4498
页数:10
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