LOCALIZATION OF 12-LIPOXYGENASE MESSENGER-RNA IN CULTURED OLIGODENDROCYTES AND ASTROCYTES BY IN-SITU REVERSE-TRANSCRIPTASE AND POLYMERASE CHAIN-REACTION

被引：32

作者：

BENDANI, MK ^{[1
]}

PALLUY, O ^{[1
]}

COOKMOREAU, J ^{[1
]}

BENEYTOUT, JL ^{[1
]}

RIGAUD, M ^{[1
]}

VALLAT, JM ^{[1
]}

机构：

[1] FAC MED LIMOGES,NEUROL LAB,F-87100 LIMOGES,FRANCE

来源：

NEUROSCIENCE LETTERS | 1995年 / 189卷 / 03期

关键词：

OLIGODENDROCYTES; ASTROCYTES; LIPOXYGENASE; IN SITU REVERSE TRANSCRIPTASE; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0304-3940(95)11482-C

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We studied the distribution of 12-lipoxygenase mRNA in glial cells. First, mRNA was detected from cellular extracts by soluble-phase reverse transcriptase-polymerase chain reaction (RT-PCR). Taking into account that cell culture populations could not be 100% homogenous, we then developed, for the first time, an in situ RT-PCR combined with immunocytochemistry with cell specific markers. Using this procedure we showed that 12-lipoxygenase mRNA was expressed both in mature oligodendrocytes and astrocytes.

引用

页码：159 / 162

页数：4

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