CYTOSOLIC CALCIUM TRANSIENTS - SPATIAL LOCALIZATION AND ROLE IN DROSOPHILA PHOTORECEPTOR CELL-FUNCTION

被引:121
作者
RANGANATHAN, R
BACSKAI, BJ
TSIEN, RY
ZUKER, CS
机构
[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093
[2] UNIV CALIF SAN DIEGO,DEPT NEUROSCI,LA JOLLA,CA 92093
[3] UNIV CALIF SAN DIEGO,DEPT PHARMACOL,LA JOLLA,CA 92093
[4] UNIV CALIF SAN DIEGO,HOWARD HUGHES MED INST,LA JOLLA,CA 92093
关键词
D O I
10.1016/0896-6273(94)90250-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Drosophila phototransduction is a phosphoinositide-mediated and Ca2+-regulated signaling cascade ideal for the dissection of feedback regulatory mechanisms. To study the roles of intracellular Ca2+ ([Ca2+](i)) in this process, we developed novel techniques for the measurement of [Ca2+](i) in intact photoreceptors. We genetically engineered flies that express a UV-specific rhodopsin in place of the normal rhodopsin, so that long wavelength light can be used to image [Ca2+](i) changes while minimally exciting the photoreceptor cells. We show that activation with UV generates [Ca2+](i) increases that are spatially localized to the rhabdomeres and that are entirely dependent on the influx of extracellular Ca2+. Application of intracellular Ca2+ chelators of varying affinities demonstrates that the Ca2+ influx initially generates a large-amplitude transient that is crucial for negative regulation. Internal Ca2+ stores were revealed by discharging them with thapsigargin. But, in contrast to proposals that IP3-sensitive stores mediate phototransduction, thapsigargin does not mimic or acutely interfere with photoexcitation. Finally, we identify a photoreceptor-specific PKC as essential for normal kinetics of [Ca2+](i) recovery.
引用
收藏
页码:837 / 848
页数:12
相关论文
共 63 条
[1]   BIOLOGICALLY USEFUL CHELATORS THAT RELEASE CA-2+ UPON ILLUMINATION [J].
ADAMS, SR ;
KAO, JPY ;
GRYNKIEWICZ, G ;
MINTA, A ;
TSIEN, RY .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (10) :3212-3220
[2]  
Augustine G J, 1992, Curr Opin Neurobiol, V2, P302, DOI 10.1016/0959-4388(92)90119-6
[3]   CALCIUM REQUIREMENTS FOR SECRETION IN BOVINE CHROMAFFIN CELLS [J].
AUGUSTINE, GJ ;
NEHER, E .
JOURNAL OF PHYSIOLOGY-LONDON, 1992, 450 :247-271
[4]   INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].
BERRIDGE, MJ .
NATURE, 1993, 361 (6410) :315-325
[5]   ISOLATION OF A PUTATIVE PHOSPHOLIPASE-C GENE OF DROSOPHILA, NORPA, AND ITS ROLE IN PHOTOTRANSDUCTION [J].
BLOOMQUIST, BT ;
SHORTRIDGE, RD ;
SCHNEUWLY, S ;
PERDEW, M ;
MONTELL, C ;
STELLER, H ;
RUBIN, G ;
PAK, WL .
CELL, 1988, 54 (05) :723-733
[6]   SPECTRAL TUNING OF RHODOPSIN AND METARHODOPSIN IN-VIVO [J].
BRITT, SG ;
FEILER, R ;
KIRSCHFELD, K ;
ZUKER, CS .
NEURON, 1993, 11 (01) :29-39
[7]   DETECTION OF LIGHT-INDUCED-CHANGES OF INTRACELLULAR IONIZED CALCIUM CONCENTRATION IN LIMULUS VENTRAL PHOTORECEPTORS USING ARSENAZO III [J].
BROWN, JE ;
BROWN, PK ;
PINTO, LH .
JOURNAL OF PHYSIOLOGY-LONDON, 1977, 267 (02) :299-320
[8]   CHANGES IN INTRACELLULAR FREE CALCIUM CONCENTRATION DURING ILLUMINATION OF INVERTEBRATE PHOTORECEPTORS - DETECTION WITH AEQUORIN [J].
BROWN, JE ;
BLINKS, JR .
JOURNAL OF GENERAL PHYSIOLOGY, 1974, 64 (06) :643-665
[9]  
BROWN JE, 1986, MOL MECH PHOTORECEPT, P231
[10]   CHEMOTAXIS OF NEWT EOSINOPHILS - CALCIUM REGULATION OF CHEMOTACTIC RESPONSE [J].
BRUNDAGE, RA ;
FOGARTY, KE ;
TUFT, RA ;
FAY, FS .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (06) :C1527-C1543