NEW TETRASACCHARIDE FLAVONOL GLYCOSIDE FROM EPIMEDIUM-ACUMINATUM

A new tetrasaccharide flavonol glycoside was isolated from the aerial parts of Epimedium acuminatum, along with three known flavonoids. The structure of the new compound, named acuminatoside [1], was established to be anhydroicaritin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-alpha-L-rhamnopyranoside-7-O-beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucopyranoside by means of spectroscopic techniques (uv, eims, fdms, fabms, H-1 nmr, H-1-H-1 COSY, 2D-J, C-13 nmr, APT, and H-1-C-13 HETCOR) and chemical methods (acid hydrolysis, enzymatic hydrolysis, and tlc-densitometry). The known compounds were identified as icariin, epimedoside A, and kaempferitrin.