PRODUCTION AND CHARACTERIZATION OF ANTIBODY AGAINST AFLATOXIN G1

被引：21

作者：

CHU, FS ^{[1
]}

STEINERT, BW ^{[1
]}

GAUR, PK ^{[1
]}

机构：

[1] UNIV WISCONSIN, DEPT FOOD MICROBIOL & TOXICOL, MADISON, WI 53706 USA

来源：

JOURNAL OF FOOD SAFETY | 1985年 / 7卷 / 03期

关键词：

D O I：

10.1111/j.1745-4565.1985.tb00539.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Antibody against aflatoxin G1 (AFG1) was obtained from rabbits after immunizing the animals with AFG1 hemiacetal (AFG2a) conjugated to bovine serum albumin. A direct heterogenous ELISA in which AFG2a was conjugated to horseradish peroxidase was used for monitoring the antibody titers and for toxin detection. Competitive ELISA assay revealed that the antibody was most specific for AFG2a and least for AFB2. The relative cross-reactivity of this antiserum with aflatoxins G2a, G2, G1, M1, B1 and B2 was found to be 1, 7, 13, 47, 48, and 63, respectively. The lower detection limits for detection of AFG1 after derivatizing to AFG2a was around 15-25 pg per assay.

引用

页码：161 / 170

页数：10

共 26 条

[1] INTERACTION OF AFLATOXIN B2A WITH AMINO-ACIDS AND PROTEINS
ASHOOR, SH
CHU, FS
[J]. BIOCHEMICAL PHARMACOLOGY, 1975, 24 (19) : 1799 - 1805
[2] REDUCTION OF AFLATOXIN-B2A WITH SODIUM-BOROHYDRIDE
ASHOOR, SH
CHU, FS
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1975, 23 (03) : 445 - 447
[3] BIERMANN A, 1980, ARCH LEBENSMITTELHYG, V31, P51
[4] BUSBY WF, 1982, MYCOTOXINS N NITROSO, V2, P3
[5] Butler W. H., 1974, Mycotoxins,, P1
[6] IMMUNOASSAYS FOR ANALYSIS OF MYCOTOXINS
CHU, FS
[J]. JOURNAL OF FOOD PROTECTION, 1984, 47 (07) : 562 - 569
[7] PRODUCTION OF ANTIBODY AGAINST AFLATOXIN-B1
CHU, FS
UENO, I
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 33 (05) : 1125 - 1128
[8] CHU FS, 1971, J ASSOC OFF ANA CHEM, V54, P1304
[9] ELNAKIB O, 1981, J ASSOC OFF ANA CHEM, V64, P1077
[10] PRODUCTION AND CHARACTERIZATION OF ANTIBODY AGAINST AFLATOXIN-Q1
FAN, TSL
ZHANG, GS
CHU, FS
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (03) : 526 - 532

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