SYNTHESIS OF A BIOSPECIFIC ADSORBENT FOR THE PURIFICATION OF THE 3 HUMAN RETINOIC ACID RECEPTORS BY AFFINITY-CHROMATOGRAPHY

被引：7

作者：

BOURGUET, W

SABLONNIERE, B

FORMSTECHER, P

CHEN, JY

BERNIER, JL

HENICHART, JP

机构：

[1] LAB BIOCHIM PROT,INSERM,U16,PL VERDUN,F-59045 LILLE,FRANCE

[2] FAC MED LILLE,BIOCHIM STRUCT LAB,F-59045 LILLE,FRANCE

[3] FAC MED STRASBOURG,CNRS,LGME,INSERM,U184,F-67085 STRASBOURG,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 187卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91253-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The total synthesis of an affinity gel suitable for the purification of retinoic acid receptors (hRARs) is reported. A chalcone derived from a potent retinobenzoic acid (Ch55) was chosen as the ligand and fixed to an immobilized matrix by coupling with the N-hydroxysuccinimide ester of agarose (Affi-Gel 10, Bio-Rad Laboratories). Efficiencies of purification of the different human RARs were tested, using recombinant receptors produced with the baculovirus expression system. © 1992.

引用

页码：711 / 716

页数：6

共 24 条

[1] A NEW RETINOIC ACID RECEPTOR IDENTIFIED FROM A HEPATOCELLULAR-CARCINOMA [J].