AUTOCRINE INHIBITION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR BY INTRACELLULAR EXPRESSION OF A SINGLE-CHAIN ANTIBODY

被引：41

作者：

BEERLI, RR

WELS, W

HYNES, NE

机构：

[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND

[2] TUMOR BIOL CTR,D-79106 FREIBURG,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2511

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene encoding a single-chain antibody which specifically binds the human epidermal growth factor (EGF) receptor has been constructed and expressed intracellularly. The single-chain antibody is derived from monoclonal antibody 225 which competes with EGF for binding to the extracellular domain of the receptor. The single-chain antibody was provided with a signal peptide to direct it to the secretory pathway and was expressed in EGF receptor transformed NIH/3T3 fibroblasts. EGF induced activation of its receptor was reduced in these cells. In addition, EGF-induced anchorage-independent growth of the cells was inhibited. The data suggest that the single-chain antibody functions in an autocrine fashion to inhibit the activity of the EGF receptor. (C) 1994 Academic Press, Inc.

引用

页码：666 / 672

页数：7

共 23 条

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