INVOLVEMENT OF ENDOGENOUS NITRIC-OXIDE IN THE REGULATION OF RAT INTESTINAL MOTILITY INVIVO

The effect of the nitric oxide (NO) synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME) on the motility of the small intestine in an acute model in the anaesthetised rat was determined by changes in jejunal intraluminal pressure. L-NAME (0.5-10 mg kg-1 i.v.) caused a dose-dependent increase in intraluminal pressure and initiated phasic intestinal contractions. These responses were inhibited by concurrent administration of L-arginine (200 mg kg-1 i.v.) but not by D-arginine (200 mg kg-1). The increase in jejunal motility induced by L-NAME was attenuated by atropine (4 mg kg-1), although even high doses of atropine (16 mg kg-1) did not abolish these responses. This indicates that although there are interactions between NO and muscarinic cholinergic mechanisms, other processes are also involved in these contractile events following administration of L-NAME. These observations in the rat suggest that endogenous NO plays a role in the modulation of intestinal motility in vivo.