ADENOVIRUS MYOCARDITIS - RETROSPECTIVE DIAGNOSIS BY GENE AMPLIFICATION FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUES

被引：27

作者：

LOZINSKI, GM

DAVIS, GG

KROUS, HF

BILLMAN, GF

SHIMIZU, H

BURNS, JC

机构：

[1] UNIV CALIF SAN DIEGO,SCH MED,DEPT PEDIAT,LA JOLLA,CA 92093

[2] UNIV CALIF SAN DIEGO,SCH MED,DEPT PATHOL,LA JOLLA,CA 92093

[3] SAN DIEGO CTY MED EXAMINERS OFF,SAN DIEGO,CA

[4] CHILDRENS HOSP,DEPT PATHOL,SAN DIEGO,CA

[5] CHILDRENS HOSP,DEPT PEDIAT,SAN DIEGO,CA

来源：

HUMAN PATHOLOGY | 1994年 / 25卷 / 08期

关键词：

POLYMERASE CHAIN REACTION; GENE AMPLIFICATION; ADENOVIRUS; MYOCARDITIS; VIRAL DIAGNOSIS;

D O I：

10.1016/0046-8177(94)90255-0

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Understanding the pathogenesis of viral myocarditis is linked to the availability of sensitive assays to detect viruses in clinical material. Recent advances in molecular techniques permit direct detection of viral-specific nucleic acid in tissue samples. This report describes a protocol for DNA extraction and amplification of adenovirus genome from formalin-fixed, paraffin-embedded human tissues that detects as little as 10 copies of viral genome in a background of 0.5 μg of human DNA. This sensitive assay permitted the examination of archived tissues to establish a retrospective diagnosis of adenoviral myocarditis in two pediatric patients. © 1994.

引用

页码：831 / 834

页数：4

共 20 条

[1] POLYMERASE CHAIN-REACTION FOR DETECTION OF ADENOVIRUSES IN STOOL SAMPLES [J].