HALOPHILIC PROTEIN STABILIZATION BY THE MILD SOLUBILIZING AGENTS NONDETERGENT SULFOBETAINES

被引：23

作者：

VUILLARD, L

MADERN, D

FRANZETTI, B

RABILLOUD, T

机构：

[1] INST BIOL STRUCT,F-38027 GRENOBLE 1,FRANCE

[2] CEN GRENOBLE,BMCC,DBMS,INSERM,F-38054 GRENOBLE 9,FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 230卷 / 02期

关键词：

D O I：

10.1006/abio.1995.1476

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separation of halophilic bacteria elongation factor Tu and halophilic malate dehydrogenase by high-performance ion-exchange chromatography achieved at reduced salt levels without significant loss of activity, (C) 1995 Academic Press, Inc.

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页码：290 / 294

页数：5

相关论文

共 19 条

[1] PREFERENTIAL INTERACTIONS OF PROTEINS WITH SOLVENT COMPONENTS IN AQUEOUS AMINO-ACID SOLUTIONS [J].

ARAKAWA, T ;

TIMASHEFF, SN .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 224 (01) :169-177

[2] STABILIZATION OF PROTEIN-STRUCTURE BY SUGARS [J].

ARAKAWA, T ;

TIMASHEFF, SN .

BIOCHEMISTRY, 1982, 21 (25) :6536-6544

[3] THE STABILIZATION OF PROTEINS BY OSMOLYTES [J].

ARAKAWA, T ;

TIMASHEFF, SN .

BIOPHYSICAL JOURNAL, 1985, 47 (03) :411-414

[4] PREFERENTIAL INTERACTIONS DETERMINE PROTEIN SOLUBILITY IN 3-COMPONENT SOLUTIONS - THE MGCL2 SYSTEM [J].

ARAKAWA, T ;

BHAT, R ;

TIMASHEFF, SN .

BIOCHEMISTRY, 1990, 29 (07) :1914-1923

[5]

CADENAS Q, 1994, BIOCHEM MOL BIOL INT, V33, P785

[6] PURIFICATION AND PROPERTIES OF A HALOPHILIC CATALASE PEROXIDASE FROM HALOARCULA-MARISMORTUI [J].

CENDRIN, F ;

JOUVE, HM ;

GAILLARD, J ;

THIBAULT, P ;

ZACCAI, G .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1994, 1209 (01) :1-9

[7] CLONING, SEQUENCING, AND EXPRESSION IN ESCHERICHIA-COLI OF THE GENE CODING FOR MALATE-DEHYDROGENASE OF THE EXTREMELY HALOPHILIC ARCHAEBACTERIUM HALOARCULA-MARISMORTUI [J].

CENDRIN, F ;

CHROBOCZEK, J ;

ZACCAI, G ;

EISENBERG, H ;

MEVARECH, M .

BIOCHEMISTRY, 1993, 32 (16) :4308-4313

[8] SOLUTION STUDIES OF ELONGATION-FACTOR TU FROM THE EXTREME HALOPHILE HALOBACTERIUM-MARISMORTUI [J].

EBEL, C ;

GUINET, F ;

LANGOWSKI, J ;

URBANKE, C ;

GAGNON, J ;

ZACCAI, G .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (01) :361-371

[9] BIOCHEMICAL, STRUCTURAL, AND MOLECULAR-GENETIC ASPECTS OF HALOPHILISM [J].

EISENBERG, H ;

MEVARECH, M ;

ZACCAI, G .

ADVANCES IN PROTEIN CHEMISTRY, 1992, 43 :1-62

[10] STABILIZATION OF CALF-BRAIN MICROTUBULE PROTEIN BY SUCROSE [J].

FRIGON, RP ;

LEE, JC .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1972, 153 (02) :587-589