MICRODISSECTION OF THE Y-CHROMOSOME AND FLUORESCENCE IN-SITU HYBRIDIZATION ANALYSIS OF THE SEX-CHROMOSOMES OF LAKE TROUT, SALVELINUS-NAMAYCUSH

被引：29

作者：

REED, KM ^{[1
]}

BOHLANDER, SK ^{[1
]}

PHILLIPS, RB ^{[1
]}

机构：

[1] UNIV CHICAGO,HEMATOL ONCOL SECT,CHICAGO,IL 60637

来源：

CHROMOSOME RESEARCH | 1995年 / 3卷 / 04期

关键词：

FLUORESCENCE IN SITU HYBRIDIZATION; LAKE TROUT; MICRODISSECTION; SEX CHROMOSOMES;

D O I：

10.1007/BF00713046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lake trout, Salvelinus namaycush, is one of the few salmonids with morphologically differentiated sex chromosomes. Genetic analysis suggested that the sex-determining region of this species lies on the short arm of the Y chromosome. The differential arm of the Y chromosome was microdissected and the resulting DNA amplified in a sequence-independent manner. Amplified DNA was biotin labeled as a probe for fluorescence in situ hybridization (FISH). Strong hybridization signals were seen covering defined regions of both the Y and X chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were not identified by FISH with the Y probe, indicating diploidization of this region of the genome.

引用

页码：221 / 226

页数：6

共 30 条

[1]

Allendorf F.W., Gellman W.A., Thorgaard G.H., Sex-linkage of two enzyme loci in rainbow trout, Heredity, 72, pp. 498-507, (1994)

[2]

Allendorf F.W., Thorgaard G.H., Tetraploidy and the evolution of salmonid fishes, Evolutionary Genetics of Fishes, pp. 1-53, (1984)

[3]

Allendorf F.W., Utter F.M., May B.P., Gene duplication within the family Salmonidae: detection and determination of the genetic control of duplicate loci through inheritance studies and the examination of populations, Isozymes IV. Genetics and Evolution, pp. 415-431, (1975)

[4]

Baker R.J., Wichman H.A., Retrotransposon mys is concentrated on the sex chromosomes: implications for copy number containment, Evolution, 44, pp. 2083-2088, (1990)

[5]

Bohlander S.K., Espinosa R., Le Beau M.M., Rowley J.D., Daaz M.O., A method for the rapid sequence-independent amplification of microdissected chromosomal material, Genomics, 13, pp. 1322-1324, (1992)

[6]

Bohlander S.K., Espinosa R., Fernald A., Rowley J.D., Le Beau M.M., Daaz M.O., Sequence-independent amplification of yeast artificial chromosomes for fluorescence in situ hybridization, Cytogenetic and Genome Research, 65, pp. 108-110, (1994)

[7]

Devlin R.H., McNeil B.K., Groves T.D.D., Donaldson E.M., Isolation of a Y-chromosomal DNA probe capable of determining genetic sex in chinook salmon (Oncorhynchus tshawytscha), Canadian Journal of Fisheries and Aquatic Sciences, 48, pp. 1606-1612, (1991)

[8]

Forbes S.H., Knudsen K.L., North T.W., Allendorf F.W., One of two growth hormone genes in coho salmon is sexlinked, Proc Natl Acad Sci USA, 91, pp. 1628-1631, (1994)

[9]

Gold J.R., Cytogenetics, Fish Physiology, pp. 353-405, (1979)

[10]

Hale D.W., Sex chromosomes, heterochromatin, and retrotransposon accumulation in deer mice, Evolution, 46, pp. 1955-1958, (1992)

← 1 2 3 →