CHEMICAL SYNTHESIS, EXPRESSION AND MUTAGENESIS OF A GENE ENCODING BETA-CRYPTOGEIN, AN ELICITIN PRODUCED BY PHYTOPHTHORA-CRYPTOGEA

被引：51

作者：

ODONOHUE, MJ ^{[1
]}

GOUSSEAU, H ^{[1
]}

HUET, JC ^{[1
]}

TEPFER, D ^{[1
]}

PERNOLLET, JC ^{[1
]}

机构：

[1] INRA,BIOL RHIZOSPHERE LAB,F-78026 VERSAILLES,FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 27卷 / 03期

关键词：

CRYPTOGEIN GENE; SYNTHETIC GENE; FUNGAL ELICITOR; PHYTOPHTHORA CRYPTOGEA;

D O I：

10.1007/BF00019323

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining residues. All of the highly necrotic beta-elicitins have a hydrophilic residue (usually lysine) at position 13, whereas in the less necrotic alpha-elicitins this residue is replaced by a valine. Here, we report the synthesis and expression of a gene encoding a highly necrotic elicitin, beta-cryptogein, and we show that the substitution of Lys-13 of this recombinant protein by a valine leads to a drastic alteration to the necrotic activity of the recombinant protein.

引用

页码：577 / 586

页数：10

共 29 条

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