HIGH-YIELD SYNTHESIS OF MONOGLYCERIDE BY MONOACYLGLYCEROL AND DIACYLGLYCEROL LIPASE FROM PENICILLIUM-CAMEMBERTII U-150

Glyceride synthetic action of mono- and diacylglycerol lipase (MDGL) from Penicillium camembertii U-150 was investigated. MDGL could synthesize monoglyceride as a main product and diglyceride, but not triglyceride, from oleic acid and glycerol with no positional specificity. Long-chain (C18) unsaturated and medium-chain (C10 and 12) saturated fatty acids were efficient acyl donors. The conversion (oleic acid consumption) and the composition of the formed glycerides were affected by the molar ratio of glycerol to oleic acid and the initial water content in medium. Under the optimal conditions, more than 90 wt% of monoglyceride in the formed glyceride was obtained with up to 76% of the conversion. The addition of molecular sieves increased the conversion of ca. 90% to 97.3% resulting in 74 wt% of monoglyceride, 23.3 wt% of diglyceride and 2.7 wt% of unesterified fatty acid. The conversion of diglyceride to monoglyceride was also observed by the addition of molecular sieves. Glycerolysis of fatty acid vinyl ester by MDGL yielded monoglyceride with a higher yield (90% of total peak area from TLC/FID analysis).