EFFECT OF 4-HYDROXYALKENALS ON HEPATIC PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE-PHOSPHOLIPASE-C

被引：71

作者：

ROSSI, MA ^{[1
]}

FIDALE, F ^{[1
]}

GARRAMONE, A ^{[1
]}

ESTERBAUER, H ^{[1
]}

DIANZANI, MU ^{[1
]}

机构：

[1] GRAZ UNIV,INST BIOCHEM,A-8010 GRAZ,AUSTRIA

来源：

BIOCHEMICAL PHARMACOLOGY | 1990年 / 39卷 / 11期

关键词：

D O I：

10.1016/0006-2952(90)90116-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effects of some 4-hydroxyalkenals, carbonylic products of lipid peroxidation, on hepatic phosphatidylinositol-4,5-bisphosphate (PIP2)-phospholipase C (PL-C) activity were investigated. The enzymatic activity was assayed in vitro by measuring the hydrolysis of [3H]PIP2 added as exogenous substrate to liver membranes. 4-Hydroxyhexenal (HEE), 4-hydroxyoctenal (HOE) and 4-hydroxynonenal (HNE) were able to stimulate both the basal and the GTPgammaS induced PL-C activity, whereas 4-hydroxyundecenal was inactive. HOE was the most active compound, being able to accelerate PIP2 breakdown at concentrations between 10-12 and 10-6M, while in the case of HEE the effective doses ranged from 10-11 to 10-7 M and from 10-9 to 10-6 M in the case of HNE. 4-Hydroxynonenal was able to increase also bombesin stimulated PL-C activity. As these aldehydes accelerated PIP2 breakdown at doses which can be actually reached in tissues, the effects shown in vitro are likely to occur in vivo. © 1990.

引用

页码：1715 / 1719

页数：5

共 27 条

[1]

ABDELLATIF AA, 1986, PHARMACOL REV, V38, P227

[2]

BALDASSARE JJ, 1986, J BIOL CHEM, V261, P1942

[3] IDENTIFICATION OF 4-HYDROXYNONEAL AS A CYTO-TOXIC PRODUCT ORIGINATING FROM THE PEROXIDATION OF LIVER MICROSOMAL LIPIDS [J].